Products -> EZTest® Self-Contained Biological Indicator

EZTest is a self-contained biological indicator for monitoring steam / flash steam, ethylene oxide, hydrogen peroxide, or propylene oxide sterilization. EZTest contains bacterial spores on a filter-paper carrier packaged within a small, thermoplastic culture tube. Inside the thermoplastic culture tube is a sealed-glass ampoule of specially-formulated soybean casein digest culture medium containing a color indicator which turns a dramatic yellow when spores grow. EZTest is easy-to-use and produces visual results within 24 hours (steam, hydrogen peroxide, or propylene oxide) or 48 hours (ethylene oxide) without laboratory transfers.

Availability

Use

Please follow instructions provided with each box of EZTest biological indicators.

1. Place the EZTest biological indicator in a horizontal position in the most difficult to sterilize location of the load.
2. For Flash cycles, articles should be unwrapped.
3. Place a sufficient number of EZTest biological indicators throughout the load to be sterilized. NOTE: This number will vary depending on the regulations being met but never less than two is recommended. Ten units per cycle is commonly used.
4. Expose the load to the sterilization cycle.
5. After sterilizing, send test units to the test laboratory along with at least one non- sterilized unit marked POSITIVE CONTROL.
6. Following exposure, remove the EZTest biological indicators as soon as the load cools or has been aerated.
7. Crush the glass culture medium ampoule using a SGM crusher to activate the culture system.
8. Place in appropriate incubator. EZTest Steam, H₂O₂ or PPO: 24 hours at 55-60°C.
   EZTest Gas: 48 hours at 35-39°C.
9. If the spores survive the sterilization cycle, the culture medium will turn to yellow (positive).
10. If the spores have been killed, the culture medium will remain the original color (red - ethylene oxide; purple - steam, H₂O₂ or PPO).
11. It is best to read results approximately every 12 to 24 hours.
12. All positive (yellow) samples should be recorded and discarded immediately. All yellow samples should be autoclaved prior to disposal.

Storage

Room temperature.

Expiration

24 months from date of manufacture.

Certification

EZTest biological indicators are traceable to a recognized culture collection and certified for population, D-value, Z-value and performance.

Product Highlights
FDA 510(k) Cleared (steam and EO) 24 hour results for steam, H2O2 or PPO 48 hour results for EO Easy to Culture No Laboratory Required Dramatic Color Change

Related Products

Smart-Read EZTest Biological Indicator Monitoring System offers faster biological results and automated documentation. Incubator for EZTest biological indicators EZTest® Test Pack process challenge devices for ethylene oxide or steam featuring EZTest biological indicators Releasat® rapid readout culturing kit for ethylene oxide featuring SGMStrip biological indicators SGMStrip™ conventional paper strip biological indicators for steam and ethylene oxide

Purchase
Purchase SGM Biotech Products

EZTest Documents
Material Safety Data Sheets Technical Reports Sample Certificates Product Manuals Spore News Technical Papers Technical Data Sheets

Top EZTest FAQs

At what temperatures does SGM perform D-value studies on EZTest steam? I need to validate sterilization of a steam piping system. What type of biological indicator should I use and how do I secure the BIs at various locations? I sent biological indicators out for a third party verification of the D-value and the results were greater than +/- 20% of the stated value on the Certificate of Analysis. Does this mean I have to reject this lot of product? My EZTest steam biological indicator was positive. I expected it to be killed. What’s wrong with this biological indicator? The end of the incubation period for my biological indicators falls on the weekend.  Can I skip that reading and score the units on the next business day?